Journal: bioRxiv

Article Title: MgAl-layered double hydroxide nanoparticles mediated oral delivery of dsRNA silencing jhp and chi genes with sublethal effects in Phthorimaea absoluta

doi: 10.1101/2025.10.17.683103

Figure Lengend Snippet: Survival analysis of Phthorimaea absoluta larvae across the different treatments. jhp-dsRNA (juvenile hormone inducible protein) ; chi-dsRNA ( chitin synthase A); egfp-dsRNA (enhanced green fluorescent protein ); MgAl-LDH: Magnesium aluminum-layered double hydroxide nanoparticles. Each treatment group for the survival rate experiment had 30 individuals. Survival curves were analyzed using the Kaplan-Meier method, with differences assessed by the log-rank test. ns: no significant difference.

Article Snippet: To clone the target genes, chi and jhp , total RNA was extracted from third and fourth P. absoluta instar larvae using the NEB Monarch Spin RNA extraction kit (NEB, T2110) following the manufacturer’s instructions.

Techniques:

Journal: bioRxiv

Article Title: MgAl-layered double hydroxide nanoparticles mediated oral delivery of dsRNA silencing jhp and chi genes with sublethal effects in Phthorimaea absoluta

doi: 10.1101/2025.10.17.683103

Figure Lengend Snippet: Effect of naked and BioClay TM dsRNAs on Phthorimaea absoluta pupation and pupal weight (mg). A. Larvae that reached the pupal stage. B. Pupal weight. Asterisks denote the level of significance: * P < 0.05, ** P < 0.01 (one-way ANOVA followed by Tukey’s multiple comparisons). ns . no significant difference.

Article Snippet: To clone the target genes, chi and jhp , total RNA was extracted from third and fourth P. absoluta instar larvae using the NEB Monarch Spin RNA extraction kit (NEB, T2110) following the manufacturer’s instructions.

Techniques:

Journal: bioRxiv

Article Title: MgAl-layered double hydroxide nanoparticles mediated oral delivery of dsRNA silencing jhp and chi genes with sublethal effects in Phthorimaea absoluta

doi: 10.1101/2025.10.17.683103

Figure Lengend Snippet: Effect of naked and BioClay TM dsRNAs on Phthorimaea absoluta adult emergence. A. Adult emergence. B. Phenotypes of failed P. absoluta adults. Asterisks denote the level of significance: * P < 0.05, ** P < 0.01 (one-way ANOVA followed by Tukey’s multiple comparisons).

Article Snippet: To clone the target genes, chi and jhp , total RNA was extracted from third and fourth P. absoluta instar larvae using the NEB Monarch Spin RNA extraction kit (NEB, T2110) following the manufacturer’s instructions.

Techniques:

Journal: bioRxiv

Article Title: MgAl-layered double hydroxide nanoparticles mediated oral delivery of dsRNA silencing jhp and chi genes with sublethal effects in Phthorimaea absoluta

doi: 10.1101/2025.10.17.683103

Figure Lengend Snippet: Chi gene mRNA level in Phthorimaea absoluta . Expression levels normalized to EF1-α gene. A) after 24 h post-feeding, B) after 48 h, and C) after 72 h. Error bars represent standard error of the mean of 3 technical replicates (5 insects per replicate). Each black dot represents a single data point (observation). Significant differences between means (multiple t-tests) are depicted.

Article Snippet: To clone the target genes, chi and jhp , total RNA was extracted from third and fourth P. absoluta instar larvae using the NEB Monarch Spin RNA extraction kit (NEB, T2110) following the manufacturer’s instructions.

Techniques: Expressing

Journal: bioRxiv

Article Title: MgAl-layered double hydroxide nanoparticles mediated oral delivery of dsRNA silencing jhp and chi genes with sublethal effects in Phthorimaea absoluta

doi: 10.1101/2025.10.17.683103

Figure Lengend Snippet: Jhp gene mRNA level in Phthorimaea absoluta . Expression levels normalized to EF1 - α gene. A) after 24 h post-feeding, B) after 48 h, and C) after 72 h. Error bars represent standard error of the mean of 3 technical replicates (5 insects per replicate). Each black dot represents a single data point (observation). Significant differences between means (multiple t-tests) are depicted.

Article Snippet: To clone the target genes, chi and jhp , total RNA was extracted from third and fourth P. absoluta instar larvae using the NEB Monarch Spin RNA extraction kit (NEB, T2110) following the manufacturer’s instructions.

Techniques: Expressing